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GLP-1 activates the GLP-1 receptor (GLP-1R) in a glucose-dependent manner to potentiate insulin release and suppress glucagon secretion, and thereby lead to blood sugar lowering and weight loss [3][4]

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The sections were then rinsed with PBS and treated with Protease IV (Advanced Cell Diagnostics (ACD), Hayward, CA) for 30 min at RT (followed by two 1 min rinses in PBS)