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Avoid requiring human studies when possible

Harmine was also obtained from the dried roots of Sophora tonkinensis Gagnep., a herb used in traditional Chinese medicine, through ethanol extraction at room temperature and acidbase liquidliquid extraction, followed by column chromatography (Wu et al

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Supernatant was aliquoted to fresh tubes in equal amounts (400 l) and frozen at 80C until further procedures

M.BruemmerD.et al (2023)