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Using a microplate reader at 488 nm excitation wavelength and 525 nm emission wavelength, the intensity of sample fluorescence was detected in real time, and changes in intracellular reactive oxygen species content were determined by comparing with the blank group ( 2.4 Lipid peroxidation assay Lipid peroxidation was determined by measuring the levels of malondialdehyde (MDA) as described in the Lipid Peroxidation MDA Detection Kit (Beyotime Biotech, China)

IGF-1 levels rose by 1.5 to 3-fold and remained elevated for 9 to 11 days after a single injection

Toll-like receptors

Wellington, New Zealand: MoH, 2011

(etc) [DOI] [PubMed] [Google Scholar] 184.Ohno M., Nishida A