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All tests were two-tailed, and results were considered statistically significant if p-values were less than 0.05

Full detox from nicotine is achieved within 4 to 6 weeks, after which many former smokers report a marked decrease in cravings and a substantial improvement in overall well-being

Acknowledgments The authors express gratitude to the NGS facility and Mass facility at IISER Bhopal for facilitating the sequencing and mass spectrometry experiments