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The TMV-GFP-1056 plasmid was linearized at the Kpn I site and the recombinant vector transcribed using T7 RNA polymerase and the mMessage mMachine kit (Ambion), following the protocol of the manufacturer

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Finally, our use of a 5-ppm CO threshold for abstinence was important because reducers may have CO values between this value and the usual 10-ppm threshold and may be incorrectly counted as abstainers if the 10ppm threshold is adopted

These include Sidi Abd El-Rahman in Matruh and Alexandria Governorate within a 25-mile radius