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Cells were stimulated with live or heat-killed bacteria (MOI 10:1), reporter activity was quantified using colorimetric and luminescent assays, immunofluorescence microscopy assessed macrophage interactions, and western blotting evaluated IB phosphorylation and degradation

Other symptoms may include: Flu-like illness with fever Unexplained fatigue Weight loss Wheezing Eye, skin, mouth, or nose irritation, if caused by chemical exposure Popcorn lung is often misdiagnosed as asthma, bronchitis, or emphysema

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