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Artisan tubes, on the other hand, are more like pre-rolled cones, made with ultra-thin rolling paper and featuring a spiral paper filter that traps particles and resin but is sturdy enough not to crush between your fingers

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The nicotine content was determined with an ultravioletvisible light (UV-VIS) spectrophotometer (Varian, Cary 50) at the wavelengths of maximum absorption (236, 259 and 282 nm)

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Using antibodies directed against the NF-kappaB/AP-1 p65 or c-jun AP-1 subunits and horseradish peroxidase (HRP), a secondary antibody, activated NF-kappa B or AP-1 was detected colorimetrically using a spectrophotometer