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All candidate protein sequences were then submitted to the HMMER website for the identification of the conserved protein kinase (PK) domain (PF00069) and the wall-associated receptor kinase galacturonan-binding (WAK_GUB) domain (PF13947) ( was used to calculate the theoretical molecular weight (MW) and isoelectric point (pI) of each encoded protein ( was used to predict the subcellular localizations ( 2.3 Phylogenetic relationship, gene structure and conserved motif analysis A multiple sequence alignment of WAK proteins from tobacco and Arabidopsis was performed using ClustalX 2.1 ( WAK genes

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