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After the acute treatment, the cells were washed with PBS and incubated with 25 L of the fluorescence dye 5-(and-6)-chloromethyl 2,7-dichlorodihydrofluorescein diacetate (CM-H 2 DCFDA) (Life Technologies, Oregon, USA) in 1 ml of PBS at 37 C away from light

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Avoid prolonged use For slightly more powerful devices, avoid prolonged use and turn them off after 15 minutes of operation

The significance of protecting youth for human capital development Protecting children from tobacco use is vital for supporting human capital development

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