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1 h prior to the assay time point, the medium was exchanged to the Seahorse XF Base Medium (Agilent Technologies) with 1 mM sodium pyruvate, 2 mM l-glutamine and 10 mM d-(+)-glucose (all Sigma-Aldrich), pH 7.4 and incubated at 37 C without CO 2

In extreme cases, well evaluate whether additional servicessuch as engine or cabin air system cleaningare needed for best results